The 'Whos are a race of microscopic beings who come in many different sizes, ranging from tall to small. They all live in the equally microscopic world of Whoville located in a dust speck on a flower on the top of Mt. Nool (or inside the snowflake on your sleeve). They are an extremely cheerful race that enjoys celebrations such as Christmas, where they celebrate the Whobilation (a big Christmas celebration) and the Who Centennial (A ceremony celebrating 100 years of peace and harmony in Whoville). The Whos and Whoville itself appear in the books Horton Hears a Who! and How the Grinch Stole Christmas! and also in the 1977 television special, Grinch Night.
In the books and cartoons, Whos are notably humanoid, and apart from their size (which can vary greatly) would pass as humans easily.
In the Horton Hears a Who film, Whos are furry, have short legs, long arms, a squash-shaped torso, and a decidedly not human face. Their feet are toeless and pointed, and they have four fingers on each hand. Overall, they bear a resemblance to the Grinch's TV design. They also appear to be slightly elastic, as demonstrated by Mayor McDodd and his son JoJo. Their eye color tends to match the dominant color of their fur.
Whos appear to have an incredible sense of balance, as observed by Whoville's bizarre architecture and JoJo's dexterity, among other things. In Horton Hears a Who they also seem to be incredibly flexible.
The most notable Whos within Whoville are its mayor Ned McDodd, who is the first Who to gain contact with the elephant Horton, Dr. Hoovey (whose role in the cartoon is split between Ned and Dr. LaRue), the young and very small Cindy Lou Who, the brave Euchariah Who, who foiled the Grinch on Halloween (also called Grinch Night) in the cartoon with the same title by overcoming his fears, and the once silent JoJo, the mayor's son, who breaks Whoville's sound barrier in the dust speck that allowed the animals in the Jungle of Nool to hear the Whos.
- Contrary to popular belief, Whos do not speak in rhyme all the time.